Recombinant Human TUBA3D 293 Cell Lysate

• Cat.No.: TUBA3D-658HCL

Specification

• Description: Antigen standard for tubulin, alpha 3d (TUBA3D) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: TUBA3D tubulin, alpha 3d [ Homo sapiens ]
• Official Symbol: TUBA3D
• Synonyms: TUBA3D; tubulin, alpha 3d; tubulin alpha-3C/D chain; alpha tubulin isotype H2 alpha; H2 ALPHA; tubulin alpha-2 chain; alpha-tubulin isotype H2-alpha; TUBA3C; H2-ALPHA
• Gene ID: 113457
• mRNA Refseq: NM_080386
• Protein Refseq: NP_525125
• UniProt ID: Q13748
• Chromosome Location: 2q21.1
• Pathway: Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem; Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Metabolism of proteins, organism-specific biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem