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Recombinant Human TPH1 293 Cell Lysate

Cat.No. : TPH1-847HCL
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  • Gene Information
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Description : Antigen standard for tryptophan hydroxylase 1 (TPH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TPH1 tryptophan hydroxylase 1 [ Homo sapiens ]
Official Symbol : TPH1
Synonyms : TPH1; tryptophan hydroxylase 1; TPH, TPRH, tryptophan hydroxylase (tryptophan 5 monooxygenase); tryptophan 5-hydroxylase 1; tryptophan 5 monooxygenase; L-tryptophan hydroxylase; tryptophan 5-monooxygenase 1; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); TPRH; TRPH; MGC119994;
Gene ID : 7166
mRNA Refseq : NM_004179
Protein Refseq : NP_004170
MIM : 191060
UniProt ID : P17752
Chromosome Location : 11p15.3-p14
Pathway : Amine-derived hormones, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Melatonin biosynthesis, tryptophan =>serotonin => melatonin, organism-specific biosystem; Melatonin biosynthesis, tryptophan => serotonin =>

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is TPH1 protein measured in clinical practice? 02/14/2023

TPH1 protein can be measured in clinical practice using techniques such as enzyme-linked immunosorbent assay (ELISA) or immunohistochemistry.

How does TPH1 protein contribute to the regulation of circadian rhythm? 11/22/2021

TPH1 protein contributes to the regulation of circadian rhythm by influencing the synthesis of serotonin, which in turn affects mood, sleep, and behavior.

How is TPH1 protein involved in the gut-brain axis? 02/13/2019

TPH1 protein is involved in the gut-brain axis by influencing serotonin production in the gastrointestinal tract, which in turn can affect mood and behavior.

What are the implications of TPH1 polymorphisms in clinical settings? 07/05/2017

TPH1 polymorphisms have implications in clinical settings as they are associated with variations in serotonin levels and susceptibility to psychiatric disorders.

What are the potential clinical applications of TPH1 inhibitors? 02/27/2017

TPH1 inhibitors have potential clinical applications in the treatment of psychiatric disorders, gastrointestinal disorders, and migraine.

Customer Reviews (3)

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05/13/2022

    the TPH1 protein stands out for its exceptional quality and its ability to fulfill my experimental requirements.

    04/14/2016

      Its compatibility with various methodologies further enhances its value as a research tool, making it a go-to choice for investigating TPH1 protein function and molecular interactions.

      02/13/2016

        The versatility of the TPH1 protein extends beyond these applications, enabling its use in a wide range of experimental techniques.

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