"TP53I3" Related Products

Recombinant Human TP53I3 293 Cell Lysate

Cat.No.: TP53I3-857HCL
Description: Antigen standard for tumor protein p53 inducible protein 3 (TP53I3), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TP53I3 tumor protein p53 inducible protein 3 [ Homo sapiens ]
Official Symbol: TP53I3
Synonyms: TP53I3; tumor protein p53 inducible protein 3; quinone oxidoreductase PIG3; PIG3; p53-induced gene 3 protein; quinone oxidoreductase homolog;
Gene ID: 9540
mRNA Refseq: NM_001206802
Protein Refseq: NP_001193731
MIM: 605171
UniProt ID: Q53FA7
Chromosome Location: 2p23.3
Pathway: Direct p53 effectors, organism-specific biosystem; p53 signaling pathway, organism-specific biosystem; p53 signaling pathway, conserved biosystem;

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