"TFB2M" Related Products

Recombinant Human TFB2M 293 Cell Lysate

Cat.No.: TFB2M-1135HCL
Description: Antigen standard for transcription factor B2, mitochondrial (TFB2M), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: TFB2M transcription factor B2, mitochondrial [ Homo sapiens ]
Official Symbol: TFB2M
Synonyms: TFB2M; transcription factor B2, mitochondrial; dimethyladenosine transferase 2, mitochondrial; FLJ22661; FLJ23182; Hkp1; hTFB2M; h-mtTFB; h-mtTFB2; HCV NS5A-transactivated protein 5; mitochondrial 12S rRNA dimethylase 2; mitochondrial transcription factor B2; hepatitis C virus NS5A-transactivated protein 5; S-adenosylmethionine-6-N, N-adenosyl(rRNA) dimethyltransferase 2; mtTFB2;
Gene ID: 64216
mRNA Refseq: NM_022366
Protein Refseq: NP_071761
MIM: 607055
UniProt ID: Q9H5Q4
Chromosome Location: 1q44
Pathway: Energy Metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Mitochondrial Gene Expression, organism-specific biosystem; Mitochondrial transcription initiation, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem; Transcription from mitochondrial promoters, organism-specific biosystem;

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