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Recombinant Human TERT 293 Cell Lysate

Cat.No. : TERT-1144HCL
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  • Gene Information
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Description : Antigen standard for telomerase reverse transcriptase (TERT), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TERT telomerase reverse transcriptase [ Homo sapiens ]
Official Symbol : TERT
Synonyms : TERT; telomerase reverse transcriptase; EST2; hEST2; TCS1; TP2; TRT; telomerase catalytic subunit; telomerase-associated protein 2; hTRT; DKCA2; DKCB4;
Gene ID : 7015
mRNA Refseq : NM_198253
Protein Refseq : NP_937983
UniProt ID : O14746
Chromosome Location : 5p15.33
Pathway : Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Extension of Telomeres, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-2 Signaling Pathway, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there genetic mutations associated with TERT that have clinical relevance? 09/03/2021

Yes, mutations in the TERT gene have been identified in certain genetic disorders. Understanding these mutations can be crucial for diagnosing and managing related conditions.

What are the challenges associated with targeting TERT for therapeutic purposes? 04/11/2021

One challenge is the potential impact on normal, healthy cells, as they also rely on TERT for telomere maintenance. Specific targeting of cancer cells without affecting normal cells is a significant hurdle.

What are the ethical considerations surrounding the manipulation of TERT for medical purposes? 01/27/2018

The ethical implications include concerns about unintended consequences, potential misuse, and ensuring equitable access to TERT-based therapies.

Can TERT expression be used as a predictive marker for treatment response in cancer patients? 03/27/2016

Research suggests that TERT expression levels may be indicative of treatment response. Patients with higher TERT expression might respond differently to certain therapies.

How does TERT contribute to aging, and are there potential anti-aging applications? 03/18/2016

TERT's role in maintaining telomeres is linked to cellular aging. Exploring ways to modulate TERT expression could have implications for anti-aging interventions.

Customer Reviews (3)

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Reviews
07/24/2021

    This existing knowledge base provides a solid foundation for designing experiments and interpreting results, ultimately enhancing the efficiency and reliability of trials involving TERT protein.

    02/02/2018

      With their support, I can confidently explore the intricacies of TERT and make significant contributions to the understanding of its functions in various biological processes.

      08/15/2017

        Extensive research has been conducted on TERT, making it relatively accessible and well-understood in terms of its structure and functions.

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