Recombinant Human SPCS3 293 Cell Lysate

• Cat.No.: SPCS3-1525HCL

Specification

• Description: Antigen standard for signal peptidase complex subunit 3 homolog (S. cerevisiae) (SPCS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SPCS3 signal peptidase complex subunit 3 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SPCS3
• Synonyms: SPCS3; signal peptidase complex subunit 3 homolog (S. cerevisiae); signal peptidase complex subunit 3; FLJ22649; PRO3567; SPC3; SPC22/23; YLR066W; SPase 22 kDa subunit; SPase 22/23 kDa subunit; microsomal signal peptidase 23 kDa subunit; microsomal signal peptidase 22/23 kDa subunit; DKFZp564J1864
• Gene ID: 60559
• mRNA Refseq: NM_021928
• Protein Refseq: NP_068747
• UniProt ID: P61009
• Chromosome Location: 4q34.2
• Pathway: Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Gene Expression, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem
• Function: peptidase activity