"SOCS6" Related Products

Recombinant Human SOCS6 293 Cell Lysate

Cat.No.: SOCS6-1577HCL
Description: Antigen standard for suppressor of cytokine signaling 6 (SOCS6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SOCS6 suppressor of cytokine signaling 6 [ Homo sapiens ]
Official Symbol: SOCS6
Synonyms: SOCS6; suppressor of cytokine signaling 6; SOCS4, suppressor of cytokine signaling 4; CIS4; Cish4; HSPC060; SSI4; STAI4; STATI4; STAT induced STAT inhibitor-4; cytokine-inducible SH2 protein 4; suppressor of cytokine signaling 4; CIS-4; SOCS4; SOCS-4; SOCS-6;
Gene ID: 9306
mRNA Refseq: NM_004232
Protein Refseq: NP_004223
MIM: 605118
UniProt ID: O14544
Chromosome Location: 18q22
Pathway: ECS complex, organism-specific biosystem; ECS complex, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Regulation of KIT signaling, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by SCF-KIT, organism-specific biosystem;

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