"SNRNP27" Related Products

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Recombinant Human SNRNP27 293 Cell Lysate

Cat.No.: SNRNP27-1624HCL
Description: Antigen standard for small nuclear ribonucleoprotein 27kDa (U4/U6.U5) (SNRNP27) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SNRNP27 small nuclear ribonucleoprotein 27kDa (U4/U6.U5) [ Homo sapiens ]
Official Symbol: SNRNP27
Synonyms: SNRNP27; small nuclear ribonucleoprotein 27kDa (U4/U6.U5); U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein; nucleic acid binding protein RY 1; RY1; U4/U6.U5-27K; U4/U6.U5 tri-snRNP-associated protein 3; putative nucleic acid binding protein RY-1; U4/U6.U5 tri-snRNP-associated 27 kDa protein; 27K;
Gene ID: 11017
mRNA Refseq: NM_006857
Protein Refseq: NP_006848
UniProt ID: Q8WVK2
Chromosome Location: 2p14
Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U4/U6.U5 tri-snRNP, organism-specific biosystem;
Function: nucleic acid binding;

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