Recombinant Human SNAP25 293 Cell Lysate

• Cat.No.: SNAP25-1640HCL

Specification

• Description: Antigen standard for synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SNAP25 synaptosomal-associated protein, 25kDa [ Homo sapiens ]
• Official Symbol: SNAP25
• Synonyms: SNAP25; synaptosomal-associated protein, 25kDa; SNAP, synaptosomal associated protein, 25kD; synaptosomal-associated protein 25; bA416N4.2; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SUP; super protein; SNAP; RIC-4; SNAP-25; FLJ23079
• Gene ID: 6616
• mRNA Refseq: NM_003081
• Protein Refseq: NP_003072
• MIM: 600322
• UniProt ID: P60880
• Chromosome Location: 20p12-p11.2
• Pathway: Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem; BoNT Light Chain Types A, C1, E cleave SNAP-25, organism-specific biosystem; Botulinum neurotoxicity, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Dopamine Neurotransmitter Release Cycle, organism-specific biosystem; Effects of Botulinum toxin, organism-specific biosystem
• Function: SNARE binding; myosin binding; protein N-terminus binding; protein binding; protein domain specific binding; syntaxin-1 binding; voltage-gated potassium channel activity