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Recombinant Human SMURF1 293 Cell Lysate

Cat.No.: SMURF1-1647HCL
Description: Antigen standard for SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SMURF1 SMAD specific E3 ubiquitin protein ligase 1 [ Homo sapiens ]
Official Symbol: SMURF1
Synonyms: SMURF1; SMAD specific E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase SMURF1; KIAA1625; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1;
Gene ID: 57154
mRNA Refseq: NM_001199847
Protein Refseq: NP_001186776
MIM: 605568
UniProt ID: Q9HCE7
Chromosome Location: 7q21.1-q31.1
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Endocytosis, organism-specific biosystem;
Function: I-SMAD binding; R-SMAD binding; acid-amino acid ligase activity; activin binding; ligase activity; protein binding; ubiquitin-protein ligase activity;

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