"SIP1" Related Products

Recombinant Human SIP1 293 Cell Lysate

Cat.No.: SIP1-1836HCL
Description: Antigen standard for survival of motor neuron protein interacting protein 1 (SIP1), transcript variant alpha is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: GEMIN2 gem (nuclear organelle) associated protein 2 [ Homo sapiens ]
Official Symbol: SIP1
Synonyms: SIP1; SIP1-delta; gem-associated protein 2; gemin-2; component of gems 2; SMN interacting protein 1-delta; survival of motor neuron protein interacting protein 1
Gene ID: 8487
mRNA Refseq: NM_003616
Protein Refseq: NP_003607
MIM: 602595
UniProt ID: O14893
Chromosome Location: 14q13
Pathway: Gene Expression, organism-specific biosystem; RNA transport, conserved biosystem; snRNP Assembly, organism-specific biosystem
Function: protein binding
Products Types ◆ Recombinant Protein

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