"SREK1" Related Products


Recombinant Human SFRS12 293 Cell Lysate

Cat.No.: SREK1-1908HCL
Description: Antigen standard for splicing factor, arginine/serine-rich 12 (SFRS12), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SREK1 splicing regulatory glutamine/lysine-rich protein 1 [ Homo sapiens ]
Official Symbol: SREK1
Synonyms: SFRS12; SRrp86; SRrp508; splicing regulatory glutamine/lysine-rich protein 1; splicing regulatory protein 508; splicing factor, arginine/serine-rich 12; serine/arginine-rich-splicing regulatory protein 86; serine-arginine-rich splicing regulatory protein 508
Gene ID: 140890
mRNA Refseq: NM_139168
Protein Refseq: NP_631907
MIM: 609268
UniProt ID: Q8WXA9
Chromosome Location: 5q12.3
Pathway: mRNA processing, organism-specific biosystem
Function: nucleotide binding; poly(A) RNA binding

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