"SF3B3" Related Products

Recombinant Human SF3B3 293 Cell Lysate

Cat.No.: SF3B3-1916HCL
Description: Antigen standard for splicing factor 3b, subunit 3, 130kDa (SF3B3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SF3B3 splicing factor 3b, subunit 3, 130kDa [ Homo sapiens ]
Official Symbol: SF3B3
Synonyms: SF3B3; splicing factor 3b, subunit 3, 130kDa; splicing factor 3b, subunit 3, 130kD; splicing factor 3B subunit 3; KIAA0017; RSE1; SAP130; SF3b130; SAP 130; spliceosome-associated protein 130; pre-mRNA-splicing factor SF3b 130 kDa subunit; pre-mRNA splicing factor SF3b, 130 kDa subunit; STAF130;
Gene ID: 23450
mRNA Refseq: NM_012426
Protein Refseq: NP_036558
MIM: 605592
UniProt ID: Q15393
Chromosome Location: 16q22
Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U2-snRNP, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem;
Function: nucleic acid binding;

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