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Recombinant Human SERPINH1 293 Cell Lysate

Cat.No.: SERPINH1-1936HCL
Description: Antigen standard for serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SERPINH1 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) [ Homo sapiens ]
Official Symbol: SERPINH1
Synonyms: SERPINH1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); CBP1, CBP2, serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) , serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2 , SERPINH2; serpin H1; colligen; HSP47; colligin-1; colligin-2; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); CBP1; CBP2; OI10; gp46; AsTP3; PIG14; PPROM; RA-A47; SERPINH2;
Gene ID: 871
mRNA Refseq: NM_001207014
Protein Refseq: NP_001193943
MIM: 600943
UniProt ID: P50454
Chromosome Location: 11q13.5
Pathway: Endochondral Ossification, organism-specific biosystem;
Function: collagen binding; serine-type endopeptidase inhibitor activity;

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