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Recombinant Human SEPHS1 293 Cell Lysate

Cat.No.: SEPHS1-1969HCL
Description: Antigen standard for selenophosphate synthetase 1 (SEPHS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SEPHS1 selenophosphate synthetase 1 [ Homo sapiens ]
Official Symbol: SEPHS1
Synonyms: SEPHS1; selenophosphate synthetase 1; selenide, water dikinase 1; SPS; SPS1; selenium donor protein 1; selenophosphate synthase 1; selenophosphate synthetase 1 +E9; selenophosphate synthetase 1 +E9a; selenophosphate synthetase 1 major; selenophosphate synthetase 1 delta E2; selenophosphate synthetase 1 delta E8; SELD; MGC4980;
Gene ID: 22929
mRNA Refseq: NM_001195602
Protein Refseq: NP_001182531
MIM: 600902
UniProt ID: P49903
Chromosome Location: 10p14
Pathway: Metabolic pathways, organism-specific biosystem; Selenocompound metabolism, organism-specific biosystem; Selenocompound metabolism, conserved biosystem;
Function: ATP binding; GTP binding; catalytic activity; nucleotide binding; selenide, water dikinase activity; transferase activity;

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