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Recombinant Human SEC63 293 Cell Lysate

Cat.No.: SEC63-1988HCL
Description: Antigen standard for SEC63 homolog (S. cerevisiae) (SEC63) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC208436, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SEC63 SEC63 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: SEC63
Synonyms: SEC63; SEC63 homolog (S. cerevisiae); SEC63 like (S. cerevisiae); translocation protein SEC63 homolog; DNAJC23; ERdj2; PRO2507; SEC63L;
Gene ID: 11231
mRNA Refseq: NM_007214
Protein Refseq: NP_009145
MIM: 608648
UniProt ID: Q9UGP8
Chromosome Location: 6q21
Pathway: Protein export, organism-specific biosystem; Protein export, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function: heat shock protein binding; receptor activity; unfolded protein binding;

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