"SCG5" Related Products

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Recombinant Human SCG5 293 Cell Lysate

Cat.No.: SCG5-2040HCL
Description: Antigen standard for secretogranin V (7B2 protein) (SCG5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC202772, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SCG5 secretogranin V (7B2 protein) [ Homo sapiens ]
Official Symbol: SCG5
Synonyms: SCG5; secretogranin V (7B2 protein); secretory granule, neuroendocrine protein 1 (7B2 protein) , SGNE1; neuroendocrine protein 7B2; 7B2; prohormone convertase chaperone; SgV; secretogranin-5; pituitary polypeptide; secretory granule endocrine protein I; secretory granule, neuroendocrine protein 1 (7B2 protein); P7B2; SGNE1;
Gene ID: 6447
mRNA Refseq: NM_001144757
Protein Refseq: NP_001138229
MIM: 173120
UniProt ID: P05408
Chromosome Location: 15q13-q14
Function: GTP binding; enzyme inhibitor activity; protein binding; unfolded protein binding;

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