"RUNDC3A" Related Products

Recombinant Human RUNDC3A 293 Cell Lysate

Cat.No.: RUNDC3A-2113HCL
Description: Antigen standard for RUN domain containing 3A (RUNDC3A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RUNDC3A RUN domain containing 3A [ Homo sapiens ]
Official Symbol: RUNDC3A
Synonyms: RUNDC3A; RUN domain containing 3A; RUN domain-containing protein 3A; RAP2IP; RPIP8; RaP2 interacting protein 8; rap2-interacting protein 8; RPIP-8;
Gene ID: 10900
mRNA Refseq: NM_001144825
Protein Refseq: NP_001138297
MIM: 605448
UniProt ID: Q59EK9
Chromosome Location: 17q21.31
Function: small GTPase regulator activity;

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