"RPAP2" Related Products

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Recombinant Human RPAP2 293 Cell Lysate

Cat.No.: RPAP2-2238HCL
Description: Antigen standard for RNA polymerase II associated protein 2 (RPAP2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RPAP2 RNA polymerase II associated protein 2 [ Homo sapiens ]
Official Symbol: RPAP2
Synonyms: RPAP2; RNA polymerase II associated protein 2; C1orf82, chromosome 1 open reading frame 82; putative RNA polymerase II subunit B1 CTD phosphatase RPAP2; FLJ13150; RNA polymerase II-associated protein 2; C1orf82;
Gene ID: 79871
mRNA Refseq: NM_024813
Protein Refseq: NP_079089
MIM: 611476
UniProt ID: Q8IXW5
Chromosome Location: 1p22.1
Function: CTD phosphatase activity; hydrolase activity; metal ion binding; phosphoprotein phosphatase activity;

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