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Recombinant Human RORA 293 Cell Lysate

Cat.No. : RORA-2247HCL
  • Specification
  • Gene Information
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Description : Antigen standard for RAR-related orphan receptor A (RORA), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : RORA RAR-related orphan receptor A [ Homo sapiens ]
Official Symbol : RORA
Synonyms : RORA; RAR-related orphan receptor A; nuclear receptor ROR-alpha; NR1F1; ROR1; ROR2; ROR3; RZRA; ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related orphan receptor alpha; RZR-ALPHA; MGC119326; MGC119329; DKFZp686M2414;
Gene ID : 6095
mRNA Refseq : NM_002943
Protein Refseq : NP_002934
MIM : 600825
UniProt ID : P35398
Chromosome Location : 15q21-q22
Pathway : Adipogenesis, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Circadian rhythm - mammal, organism-specific biosystem; Circadian rhythm - mammal, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem;
Function : ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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