Recombinant Human ROBO1 293 Cell Lysate
Cat.No. : | ROBO1-2256HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila) [ Homo sapiens ] |
Official Symbol : | ROBO1 |
Synonyms : | ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout (axon guidance receptor, Drosophila) homolog 1; roundabout homolog 1; DUTT1; FLJ21882; SAX3; deleted in U twenty twenty; MGC131599; MGC133277; |
Gene ID : | 6091 |
mRNA Refseq : | NM_001145845 |
Protein Refseq : | NP_001139317 |
MIM : | 602430 |
UniProt ID : | Q9Y6N7 |
Chromosome Location : | 3p12.3 |
Pathway : | Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Inactivation of Cdc42 and Rac, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem; |
Function : | LRR domain binding; axon guidance receptor activity; identical protein binding; protein binding; protein heterodimerization activity; receptor activity; |
Products Types
◆ Recombinant Protein | ||
ROBO1-001H | Active Recombinant Human ROBO1 Protein, Fc-tagged | +Inquiry |
ROBO1-2415M | Recombinant Mouse ROBO1 Protein (880-1612 aa), His-tagged | +Inquiry |
Robo1-5566M | Recombinant Mouse Robo1 Protein, Myc/DDK-tagged | +Inquiry |
Robo1-17R | Recombinant Rat Robo1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ROBO1-136H | Recombinant Human ROBO1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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