"RMI1" Related Products

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Recombinant Human RMI1 293 Cell Lysate

Cat.No.: RMI1-2327HCL
Description: Antigen standard for RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RMI1 RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: RMI1
Synonyms: RMI1; RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae); C9orf76, chromosome 9 open reading frame 76; recQ-mediated genome instability protein 1; BLAP75; BLM Associated Polypeptide; 75 kDa; FLJ12888; BLM-associated protein 75 kDa; BLM-associated polypeptide, 75 kDa; homolog of yeast RecQ-mediated genome instability 1 (RMI1); FAAP75; C9orf76; RP11-346I8.1;
Gene ID: 80010
mRNA Refseq: NM_024945
Protein Refseq: NP_079221
MIM: 610404
UniProt ID: Q9H9A7
Chromosome Location: 9q22.1
Pathway: Blooms syndrome complex, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem;
Function: protein binding;

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