"RDH5" Related Products

Recombinant Human RDH5 293 Cell Lysate

Cat.No.: RDH5-2435HCL
Description: Antigen standard for retinol dehydrogenase 5 (11-cis/9-cis) (RDH5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RDH5 retinol dehydrogenase 5 (11-cis/9-cis) [ Homo sapiens ]
Official Symbol: RDH5
Synonyms: RDH5; retinol dehydrogenase 5 (11-cis/9-cis); RDH1, retinol dehydrogenase 5 (11 cis and 9 cis); 11-cis retinol dehydrogenase; HSD17B9; SDR9C5; short chain dehydrogenase/reductase family 9C; member 5; 11-cis RDH; retinol dehydrogenase 1; 9-cis-retinol specific dehydrogenase; retinol dehydrogenase 5 (11-cis and 9-cis); short chain dehydrogenase/reductase family 9C, member 5; RDH1; FLJ39337; FLJ97089;
Gene ID: 5959
mRNA Refseq: NM_001199771
Protein Refseq: NP_001186700
MIM: 601617
UniProt ID: Q92781
Chromosome Location: 12q13-q14
Pathway: Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Visual signal transduction: Cones, organism-specific biosystem; Visual signal transduction: Rods, organism-specific biosystem; Vitamin A and carotenoid metabolism, organism-specific biosystem; the visual cycle, conserved biosystem; the visual cycle, organism-specific biosystem;
Function: nucleotide binding; oxidoreductase activity; retinol dehydrogenase activity;

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