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Recombinant Human RAN 293 Cell Lysate

Cat.No.: RAN-2535HCL
Description: Antigen standard for RAN, member RAS oncogene family (RAN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RAN RAN, member RAS oncogene family [ Homo sapiens ]
Official Symbol: RAN
Synonyms: RAN; RAN, member RAS oncogene family; GTP-binding nuclear protein Ran; ARA24; Gsp1; TC4; RanGTPase; GTPase Ran; OK/SW-cl.81; ras-like protein TC4; member RAS oncogene family; ras-related nuclear protein; guanosine triphosphatase Ran; androgen receptor-associated protein 24;
Gene ID: 5901
mRNA Refseq: NM_006325
Protein Refseq: NP_006316
MIM: 601179
UniProt ID: P62826
Chromosome Location: 12q24.3
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Canonical NF-kappaB pathway, organism-specific biosystem; Disease, organism-specific biosystem; Export of Viral Ribonucleoproteins from Nucleus, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; Gene Expression, organism-specific biosystem;
Function: GTP binding; GTPase activity; androgen receptor binding; chromatin binding; nucleotide binding; protein binding; transcription coactivator activity;

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