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Recombinant Human PYGM 293 Cell Lysate

Cat.No.: PYGM-2644HCL
Description: Antigen standard for phosphorylase, glycogen, muscle (PYGM), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PYGM phosphorylase, glycogen, muscle [ Homo sapiens ]
Official Symbol: PYGM
Synonyms: PYGM; phosphorylase, glycogen, muscle; phosphorylase, glycogen; muscle; glycogen phosphorylase, muscle form; glycogen storage disease type V; McArdle syndrome; myophosphorylase;
Gene ID: 5837
mRNA Refseq: NM_001164716
Protein Refseq: NP_001158188
MIM: 608455
UniProt ID: P11217
Chromosome Location: 11q12-q13.2
Pathway: Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem;
Function: AMP binding; drug binding; glycogen phosphorylase activity; nucleotide binding; pyridoxal phosphate binding; sugar binding; transferase activity, transferring glycosyl groups;

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