"PUS1" Related Products


Recombinant Human PUS1 293 Cell Lysate

Cat.No.: PUS1-2663HCL
Description: Antigen standard for pseudouridylate synthase 1 (PUS1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PUS1 pseudouridylate synthase 1 [ Homo sapiens ]
Official Symbol: PUS1
Synonyms: PUS1; pseudouridylate synthase 1; tRNA pseudouridine synthase A, mitochondrial; tRNA uridine isomerase I; tRNA-uridine isomerase I; tRNA pseudouridylate synthase I; tRNA pseudouridine(38-40) synthase; mitochondrial tRNA pseudouridine synthase A; MLASA1; MGC11268;
Gene ID: 80324
mRNA Refseq: NM_001002019
Protein Refseq: NP_001002019
MIM: 608109
UniProt ID: Q9Y606
Chromosome Location: 12q24
Function: DNA binding; RNA binding; isomerase activity; ligand-dependent nuclear receptor transcription coactivator activity; pseudouridine synthase activity; pseudouridylate synthase activity;

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