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Recombinant Human PSMB4 293 Cell Lysate

Cat.No.: PSMB4-2772HCL
Description: Antigen standard for proteasome (prosome, macropain) subunit, beta type, 4 (PSMB4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PSMB4 proteasome (prosome, macropain) subunit, beta type, 4 [ Homo sapiens ]
Official Symbol: PSMB4
Synonyms: PSMB4; proteasome (prosome, macropain) subunit, beta type, 4; proteasome subunit beta type-4; HN3; PROS26; hsBPROS26; proteasome chain 3; macropain beta chain; proteasome beta chain; 26 kDa prosomal protein; proteasome subunit HsN3; proteasome subunit, beta type, 4; multicatalytic endopeptidase complex beta chain; HsN3; PROS-26;
Gene ID: 5692
mRNA Refseq: NM_002796
Protein Refseq: NP_002787
MIM: 602177
UniProt ID: P28070
Chromosome Location: 1q21
Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem;
Function: lipopolysaccharide binding; peptidase activity; threonine-type endopeptidase activity;

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