"PRPSAP1" Related Products

Recombinant Human PRPSAP1 293 Cell Lysate

Cat.No.: PRPSAP1-2819HCL
Description: Antigen standard for phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRPSAP1 phosphoribosyl pyrophosphate synthetase-associated protein 1 [ Homo sapiens ]
Official Symbol: PRPSAP1
Synonyms: PRPSAP1; phosphoribosyl pyrophosphate synthetase-associated protein 1; phosphoribosyl pyrophosphate synthase-associated protein 1; PAP39; PRPP synthase-associated protein 1; 39 kDa phosphoribosypyrophosphate synthase-associated protein;
Gene ID: 5635
mRNA Refseq: NM_002766
Protein Refseq: NP_002757
MIM: 601249
UniProt ID: Q14558
Chromosome Location: 17q24-q25
Function: enzyme inhibitor activity; magnesium ion binding; ribose phosphate diphosphokinase activity;

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