"PRPF3" Related Products

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Recombinant Human PRPF3 293 Cell Lysate

Cat.No.: PRPF3-2827HCL
Description: Antigen standard for PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) (PRPF3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRPF3 PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: PRPF3
Synonyms: PRPF3; PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae); PRP3 pre mRNA processing factor 3 homolog (yeast) , retinitis pigmentosa 18 (autosomal dominant) , RP18; U4/U6 small nuclear ribonucleoprotein Prp3; HPRP3; Prp3; U4/U6 snRNP 90 kDa protein; pre-mRNA-splicing factor 3; U4/U6-associated RNA splicing factor; PRP3; RP18; Prp3p; HPRP3P;
Gene ID: 9129
mRNA Refseq: NM_004698
Protein Refseq: NP_004689
MIM: 607301
UniProt ID: O43395
Chromosome Location: 1q21.1
Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U4/U6.U5 tri-snRNP, organism-specific biosystem; mRNA processing, organism-specific biosystem;
Function: protein binding;

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