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Recombinant Human PROX1 293 Cell Lysate

Cat.No. : PROX1-2830HCL
  • Specification
  • Gene Information
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Description : Antigen standard for prospero homeobox 1 (PROX1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PROX1 prospero homeobox 1 [ Homo sapiens ]
Official Symbol : PROX1
Synonyms : PROX1; prospero homeobox 1; prospero related homeobox 1; prospero homeobox protein 1; PROX-1; prospero-related homeobox 1; homeobox prospero-like protein PROX1;
Gene ID : 5629
mRNA Refseq : NM_002763
Protein Refseq : NP_002754
MIM : 601546
UniProt ID : Q92786
Chromosome Location : 1q41
Function : DBD domain binding; DNA binding; LBD domain binding; ligand-dependent nuclear receptor binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription corepressor activity; transcription regulatory region DNA binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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