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Recombinant Human PRLH 293 Cell Lysate

Cat.No.: PRLH-2843HCL
Description: Antigen standard for prolactin releasing hormone (PRLH) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRLH prolactin releasing hormone [ Homo sapiens ]
Official Symbol: PRLH
Synonyms: PRLH; prolactin releasing hormone; prolactin-releasing peptide; PRH; prolactin-releasing hormone; preproprolactin-releasing peptide; PRRP;
Gene ID: 51052
mRNA Refseq: NM_015893
Protein Refseq: NP_056977
MIM: 602663
UniProt ID: P81277
Chromosome Location: 2q37.3
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem;
Function: hormone activity; neuropeptide hormone activity; prolactin-releasing peptide receptor binding;
Products Types ◆ Recombinant Protein

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