"PRKCDBP" Related Products

Recombinant Human PRKCDBP 293 Cell Lysate

Cat.No.: PRKCDBP-2858HCL
Description: Antigen standard for protein kinase C, delta binding protein (PRKCDBP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRKCDBP protein kinase C, delta binding protein [ Homo sapiens ]
Official Symbol: PRKCDBP
Synonyms: PRKCDBP; protein kinase C, delta binding protein; cavin 3; CAVIN3; HSRBC; MGC20400; sdr related gene product that binds to c kinase; SRBC;
Gene ID: 8990
mRNA Refseq: NM_145040
Protein Refseq: NP_659477
UniProt ID: Q969G5
Chromosome Location: 11p15.4
Products Types ◆ Recombinant Protein

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