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Recombinant Human PRKACG 293 Cell Lysate

Cat.No.: PRKACG-2867HCL
Description: Antigen standard for protein kinase, cAMP-dependent, catalytic, gamma (PRKACG) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRKACG protein kinase, cAMP-dependent, catalytic, gamma [ Homo sapiens ]
Official Symbol: PRKACG
Synonyms: PRKACG; protein kinase, cAMP-dependent, catalytic, gamma; cAMP-dependent protein kinase catalytic subunit gamma; PKACg; PKA C-gamma; serine(threonine) protein kinase; KAPG;
Gene ID: 5568
mRNA Refseq: NM_002732
Protein Refseq: NP_002723
MIM: 176893
UniProt ID: P22612
Chromosome Location: 9q13
Pathway: AMPK signaling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Apoptosis, organism-specific biosystem;
Function: ATP binding; cAMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity;

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