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Recombinant Human PPP5C 293 Cell Lysate

Cat.No. : PPP5C-2908HCL
  • Specification
  • Gene Information
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Description : Antigen standard for protein phosphatase 5, catalytic subunit (PPP5C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PPP5C protein phosphatase 5, catalytic subunit [ Homo sapiens ]
Official Symbol : PPP5C
Synonyms : PPP5C; protein phosphatase 5, catalytic subunit; PPP5; serine/threonine-protein phosphatase 5; PP5; PP-T; protein phosphatase T; PPT; FLJ36922; FLJ55954;
Gene ID : 5536
mRNA Refseq : NM_001204284
Protein Refseq : NP_001191213
MIM : 600658
UniProt ID : P53041
Chromosome Location : 19q13.3
Pathway : ErbB1 downstream signaling, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem;
Function : hydrolase activity; identical protein binding; metal ion binding; protein binding; protein serine/threonine phosphatase activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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