Recombinant Human PPP2R5D 293 Cell Lysate

• Cat.No.: PPP2R5D-2915HCL

Specification

• Description: Antigen standard for protein phosphatase 2, regulatory subunit B, delta isoform (PPP2R5D), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPP2R5D protein phosphatase 2, regulatory subunit B, delta [ Homo sapiens ]
• Official Symbol: PPP2R5D
• Synonyms: PPP2R5D; protein phosphatase 2, regulatory subunit B, delta; protein phosphatase 2, regulatory subunit B (B56), delta isoform , protein phosphatase 2, regulatory subunit B, delta isoform; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; B56D; PP2A B subunit isoform B-delta; PP2A B subunit isoform R5-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A, B subunit, B delta isoform; PP2A, B subunit, R5 delta isoform; PP2A, B subunit, B56 delta isoform; PP2A, B subunit, PR61 delta isoform; protein phosphatase 2, regulatory subunit B, delta isoform; protein phosphatase 2, regulatory subunit B (B56), delta isoform; Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform; MGC2134; MGC8949
• Gene ID: 5528
• mRNA Refseq: NM_006245
• Protein Refseq: NP_006236
• MIM: 601646
• UniProt ID: Q14738
• Chromosome Location: 6p21.1
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Beta-catenin phosphorylation cascade, organism-specific biosystem; CTLA4 inhibitory signaling, organism-specific biosystem
• Function: protein binding; protein phosphatase type 2A regulator activity