"PPM1F" Related Products

Recombinant Human PPM1F 293 Cell Lysate

Cat.No.: PPM1F-2961HCL
Description: Antigen standard for protein phosphatase 1F (PP2C domain containing) (PPM1F) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPM1F protein phosphatase, Mg2+/Mn2+ dependent, 1F [ Homo sapiens ]
Official Symbol: PPM1F
Synonyms: PPM1F; protein phosphatase, Mg2+/Mn2+ dependent, 1F; protein phosphatase 1F (PP2C domain containing); protein phosphatase 1F; Ca(2+)/calmodulin dependent protein kinase phosphatase; CAMKP; CaMKPase; FEM 2; KIAA0015; partner of PIX 2; POPX2; PP2C phosphatase; protein fem-2 homolog; CaM-kinase phosphatase; Ca(2+)/calmodulin-dependent protein kinase phosphatase; FEM-2; hFEM-2;
Gene ID: 9647
mRNA Refseq: NM_014634
Protein Refseq: NP_055449
UniProt ID: P49593
Chromosome Location: 22q11.22
Function: calmodulin-dependent protein phosphatase activity; hydrolase activity; metal ion binding; phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein serine/threonine phosphatase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.