"PPIAL4A" Related Products

Recombinant Human PPIAL4A 293 Cell Lysate

Cat.No.: PPIAL4A-2974HCL
Description: Antigen standard for peptidylprolyl isomerase A (cyclophilin A)-like 4A (PPIAL4A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPIAL4A peptidylprolyl isomerase A (cyclophilin A)-like 4A [ Homo sapiens ]
Official Symbol: PPIAL4A
Synonyms: COAS2; COAS-2; PPIAL4; peptidylprolyl isomerase A (cyclophilin A)-like 4A; PPIase A-like 4A; PPIase A-like 4B; PPIase A-like 4G; cyclophilin-LC (COAS2); chromosome 1 amplified sequence 2; peptidylprolyl cis-trans isomerase A-like 4A; peptidylprolyl cis-trans isomerase A-like 4B; cyclophilin homolog overexpressed in liver cancer; peptidyl-prolyl cis-trans isomerase A-like 4A/B/C
Gene ID: 164022
mRNA Refseq: NM_178230
Protein Refseq: NP_839944
Chromosome Location: 1q21.2

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