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Recombinant Human PPAT 293 Cell Lysate

Cat.No.: PPAT-2983HCL
Description: Antigen standard for phosphoribosyl pyrophosphate amidotransferase (PPAT) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPAT phosphoribosyl pyrophosphate amidotransferase [ Homo sapiens ]
Official Symbol: PPAT
Synonyms: PPAT; phosphoribosyl pyrophosphate amidotransferase; amidophosphoribosyltransferase; GPAT; PRAT; glutamine PRPP amidotransferase; glutamine phosphoribosylpyrophosphate amidotransferase; glutamine phosphoribosylpyrophosphatate amidotransferase; ATASE;
Gene ID: 5471
mRNA Refseq: NM_002703
Protein Refseq: NP_002694
MIM: 172450
UniProt ID: Q06203
Chromosome Location: 4q12
Pathway: 5-aminoimidazole ribonucleotide biosynthesis I, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Inosine monophosphate biosynthesis, PRPP + glutamine =>IMP, organism-specific biosystem; Inosine monophosphate biosynthesis, PRPP + glutamine =>
Function: 4 iron, 4 sulfur cluster binding; amidophosphoribosyltransferase activity; metal ion binding; transferase activity, transferring glycosyl groups;

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