"PPAPDC1B" Related Products

Recombinant Human PPAPDC1B 293 Cell Lysate

Cat.No.: PPAPDC1B-2989HCL
Description: Antigen standard for phosphatidic acid phosphatase type 2 domain containing 1B (PPAPDC1B), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPAPDC1B phosphatidic acid phosphatase type 2 domain containing 1B [ Homo sapiens ]
Official Symbol: PPAPDC1B
Synonyms: DPPL1; HTPAP; phosphatidate phosphatase PPAPDC1B; diacylglycerol pyrophosphate like 1; phosphatidic acid phosphatase type 2 domain-containing protein 1B
Gene ID: 84513
mRNA Refseq: NM_032483
Protein Refseq: NP_115872
MIM: 610626
UniProt ID: Q8NEB5
Chromosome Location: 8p11.23
Pathway: Fcgamma receptor (FCGR) dependent phagocytosis, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Role of phospholipids in phagocytosis, organism-specific biosystem
Function: phosphatidate phosphatase activity

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