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Recombinant Human POT1 293 Cell Lysate

Cat.No. : POT1-3005HCL
  • Specification
  • Gene Information
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Description : Antigen standard for POT1 protection of telomeres 1 homolog (S. pombe) (POT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : POT1 protection of telomeres 1 homolog (S. pombe) [ Homo sapiens ]
Official Symbol : POT1
Synonyms : POT1; protection of telomeres 1 homolog (S. pombe); protection of telomeres protein 1; DKFZp586D211; hPot1; POT1-like telomere end-binding protein; HPOT1;
Gene ID : 25913
mRNA Refseq : NM_001042594
Protein Refseq : NP_001036059
MIM : 606478
UniProt ID : Q9NUX5
Chromosome Location : 7q31.33
Pathway : Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem; Packaging Of Telomere Ends, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem; Shelterin complex, organism-specific biosystem;
Function : DEAD/H-box RNA helicase binding; DNA binding; protein binding; single-stranded telomeric DNA binding; single-stranded telomeric DNA binding; telomerase inhibitor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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