"PON2" Related Products

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Recombinant Human PON2 293 Cell Lysate

Cat.No.: PON2-3013HCL
Description: Antigen standard for paraoxonase 2 (PON2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PON2 paraoxonase 2 [ Homo sapiens ]
Official Symbol: PON2
Synonyms: PON2; paraoxonase 2; serum paraoxonase/arylesterase 2; paraoxonase nirs; PON 2; A-esterase 2; aromatic esterase 2; serum aryldialkylphosphatase 2;
Gene ID: 5445
mRNA Refseq: NM_000305
Protein Refseq: NP_000296
MIM: 602447
UniProt ID: Q15165
Chromosome Location: 7q21.3
Pathway: Metabolic pathways, organism-specific biosystem;
Function: arylesterase activity; hydrolase activity; identical protein binding; metal ion binding;

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