"POLL" Related Products

Expand
Expand
Expand

Recombinant Human POLL 293 Cell Lysate

Cat.No.: POLL-3044HCL
Description: Antigen standard for polymerase (DNA directed), lambda (POLL) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: POLL polymerase (DNA directed), lambda [ Homo sapiens ]
Official Symbol: POLL
Synonyms: POLL; polymerase (DNA directed), lambda; DNA polymerase lambda; DNA polymerase kappa; DNA polymerase beta-2; DNA polymerase beta-N; BETAN; POLKAPPA; FLJ46002;
Gene ID: 27343
mRNA Refseq: NM_001174084
Protein Refseq: NP_001167555
MIM: 606343
UniProt ID: Q9UGP5
Chromosome Location: 10q23
Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Non-homologous end-joining, organism-specific biosystem; Non-homologous end-joining, conserved biosystem;
Function: DNA binding; DNA binding; DNA-directed DNA polymerase activity; DNA-directed DNA polymerase activity; catalytic activity; lyase activity; metal ion binding; nucleotidyltransferase activity; transferase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.