"PLEKHO1" Related Products


Recombinant Human PLEKHO1 293 Cell Lysate

Cat.No.: PLEKHO1-3110HCL
Description: Antigen standard for pleckstrin homology domain containing, family O member 1 (PLEKHO1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLEKHO1 pleckstrin homology domain containing, family O member 1 [ Homo sapiens ]
Official Symbol: PLEKHO1
Synonyms: PLEKHO1; pleckstrin homology domain containing, family O member 1; pleckstrin homology domain-containing family O member 1; CKIP 1; OC120; JBP; 2810052M02Rik; C-Jun-binding protein; CK2-interacting protein 1; casein kinase 2-interacting protein 1; PH domain-containing family O member 1; CK2 interacting protein 1; HQ0024c protein; osteoclast maturation-associated gene 120 protein; CKIP-1; RP11-458I7.3;
Gene ID: 51177
mRNA Refseq: NM_016274
Protein Refseq: NP_057358
MIM: 608335
UniProt ID: Q53GL0
Chromosome Location: 1q21.2

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