"PLEKHF1" Related Products

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Recombinant Human PLEKHF1 293 Cell Lysate

Cat.No.: PLEKHF1-3112HCL
Description: Antigen standard for pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLEKHF1 pleckstrin homology domain containing, family F (with FYVE domain) member 1 [ Homo sapiens ]
Official Symbol: PLEKHF1
Synonyms: PLEKHF1; pleckstrin homology domain containing, family F (with FYVE domain) member 1; pleckstrin homology domain-containing family F member 1; APPD; MGC4090; PHAFIN1; ZFYVE15; phafin 1; phafin-1; apoptosis-inducing protein D; PH domain-containing family F member 1; PH and FYVE domain-containing protein 1; zinc finger FYVE domain-containing protein 15; lysosome-associated apoptosis-inducing protein containing PH and FYVE domains;
Gene ID: 79156
mRNA Refseq: NM_024310
Protein Refseq: NP_077286
UniProt ID: Q96S99
Chromosome Location: 19q11
Function: metal ion binding;

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