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Recombinant Human PLEKHA8 293 Cell Lysate

Cat.No. : PLEKHA8-3115HCL
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Description : Antigen standard for pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (PLEKHA8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PLEKHA8 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 [ Homo sapiens ]
Official Symbol : PLEKHA8
Synonyms : PLEKHA8; pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8; pleckstrin homology domain-containing family A member 8; FAPP2; MGC3358; PH domain-containing family A member 8; phosphoinositol 4-phosphate adapter protein 2; serologically defined breast cancer antigen NY-BR-86; phosphatidylinositol-four-phosphate adapter protein 2; DKFZp313M1034; DKFZp686J09164;
Gene ID : 84725
mRNA Refseq : NM_001197026
Protein Refseq : NP_001183955
MIM : 608639
UniProt ID : Q96JA3
Chromosome Location : 7p21-p11.2
Function : glycolipid binding; glycolipid transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/15/2019

    Convenient usage and accurate results are offered by the protein biomolecular reagent.

    04/11/2019

      Professional technical support and prompt after-sales service are provided by the manufacturer.

      03/03/2016

        Strong stability enables the protein reagent to excel in long-term storage and performance.

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