"PLAGL1" Related Products


Recombinant Human PLAGL1 293 Cell Lysate

Cat.No.: PLAGL1-3133HCL
Description: Antigen standard for pleiomorphic adenoma gene-like 1 (PLAGL1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLAGL1 pleiomorphic adenoma gene-like 1 [ Homo sapiens ]
Official Symbol: PLAGL1
Synonyms: PLAGL1; pleiomorphic adenoma gene-like 1; zinc finger protein PLAGL1; LOT1; ZAC; LOT-1; PLAG-like 1; ZAC tumor supressor; tumor supressor ZAC; lost on transformation 1; pleiomorphic adenoma-like protein 1; pleiomorphic adenoma gene-like protein 1; ZAC1; MGC126275; MGC126276; DKFZp781P1017;
Gene ID: 5325
mRNA Refseq: NM_001080951
Protein Refseq: NP_001074420
MIM: 603044
UniProt ID: Q9UM63
Chromosome Location: 6q24-q25
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem;
Function: DNA binding; metal ion binding; nucleic acid binding; zinc ion binding;

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