"PITX2" Related Products

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Recombinant Human PITX2 293 Cell Lysate

Cat.No.: PITX2-3163HCL
Description: Antigen standard for paired-like homeodomain 2 (PITX2), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PITX2 paired-like homeodomain 2 [ Homo sapiens ]
Official Symbol: PITX2
Synonyms: PITX2; paired-like homeodomain 2; IHG2, IRID2, paired like homeodomain transcription factor 2 , RGS, RIEG, RIEG1; pituitary homeobox 2; ARP1; Brx1; IGDS; Otlx2; RS; solurshin; all1-responsive gene 1; homeobox protein PITX2; ALL1-responsive protein ARP1; paired-like homeodomain transcription factor 2; rieg bicoid-related homeobox transcription factor 1; RGS; IDG2; IHG2; PTX2; RIEG; IGDS2; IRID2; RIEG1; MGC20144; MGC111022;
Gene ID: 5308
mRNA Refseq: NM_000325
Protein Refseq: NP_000316
MIM: 601542
UniProt ID: Q99697
Chromosome Location: 4q25
Pathway: Heart Development, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem;
Function: RNA polymerase II activating transcription factor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; RNA polymerase II transcription coactivator activity; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; phosphoprotein binding; protein binding; protein homodimerization activity; ribonucleoprotein complex binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region sequence-specific DNA binding;

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