"PIK3C3" Related Products

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Recombinant Human PIK3C3 293 Cell Lysate

Cat.No.: PIK3C3-3190HCL
Description: Antigen standard for phosphoinositide-3-kinase, class 3 (PIK3C3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PIK3C3 phosphoinositide-3-kinase, class 3 [ Homo sapiens ]
Official Symbol: PIK3C3
Synonyms: PIK3C3; phosphoinositide-3-kinase, class 3; phosphatidylinositol 3-kinase catalytic subunit type 3; Vps34; PI3K type 3; PI3-kinase type 3; PtdIns-3-kinase type 3; phosphatidylinositol 3-kinase p100 subunit; VPS34; hVps34; MGC61518;
Gene ID: 5289
mRNA Refseq: NM_002647
Protein Refseq: NP_002638
MIM: 602609
UniProt ID: Q8NEB9
Chromosome Location: 18q12.3
Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem;
Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; nucleotide binding; protein binding; protein kinase activity;

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