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Recombinant Human PHKG2 293 Cell Lysate

Cat.No.: PHKG2-3222HCL
Description: Antigen standard for phosphorylase kinase, gamma 2 (testis) (PHKG2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PHKG2 phosphorylase kinase, gamma 2 (testis) [ Homo sapiens ]
Official Symbol: PHKG2
Synonyms: PHKG2; phosphorylase kinase, gamma 2 (testis); phosphorylase b kinase gamma catalytic chain, testis/liver isoform; PSK-C3; PHK-gamma-T; phosphorylase kinase subunit gamma-2; serine/threonine-protein kinase PHKG2; Phosphorylase kinase, gamma 2 (testis/liver); GSD9C;
Gene ID: 5261
mRNA Refseq: NM_000294
Protein Refseq: NP_000285
MIM: 172471
UniProt ID: P15735
Chromosome Location: 16p11.2
Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem;
Function: ATP binding; calmodulin binding; enzyme binding; nucleotide binding; phosphorylase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity;

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