"PHEX" Related Products

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Recombinant Human PHEX 293 Cell Lysate

Cat.No.: PHEX-3238HCL
Description: Antigen standard for phosphate regulating endopeptidase homolog, X-linked (PHEX) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PHEX phosphate regulating endopeptidase homolog, X-linked [ Homo sapiens ]
Official Symbol: PHEX
Synonyms: PHEX; phosphate regulating endopeptidase homolog, X-linked; HPDR, HYP, phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets); phosphate-regulating neutral endopeptidase; HPDR1; HYP1; PEX; XLH; metalloendopeptidase homolog PEX; X-linked hypophosphatemia protein; vitamin D-resistant hypophosphatemic rickets protein; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets); HYP; HPDR; LXHR;
Gene ID: 5251
mRNA Refseq: NM_000444
Protein Refseq: NP_000435
MIM: 300550
UniProt ID: P78562
Chromosome Location: Xp22.2-p22.1
Function: aminopeptidase activity; metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding;

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